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Separation of a leaf extract with column chromatography

Separation of a leaf extract with column chromatography

C2.4.4.1

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Description
Components of equipment sets
Experiment Instructions

Description

The process of chromatography was developed by Tsvet on leaf pigments at the beginning of the 20th century. Although ridiculed at first, the technique has since become one the most important methods in organic chemistry. In experiment C2.4.4.1, a leaf extract is produced and separated into its components by means of column chromatography.

Components of equipment sets

1	665 592 4	Chromatography column, 235 x 20 mm diam.
1	665 073	Dropping funnel, 75 ml, ST 29
2	664 101 4	Beaker, DURAN, 100 ml, squat
1	602 012 8	Beaker, Boro 3.3, 600 ml, tall
1	667 092 4	Mortar 30 ml, 56 mm x 36 mm
1	667 091 4	Pestle 88 mm
1	665 005 4	Funnel, Boro 3.3, 100 mm diam.
1	661 038 4	Round filter, type 615, 150 mm Ø, Set of 100
1	661 161 4	Narrow-neck glass bottle, amber glass, 100 ml
1	602 347 4	Laboratory bottle, 500 ml, GL 45 thread
1	665 754 4	Measuring cylinder 100 ml, with plastic base
1	665 756 4	Measuring cylinder 500 ml, with plastic base
1	665 217 4	Glass stirring rod, 500 mm x 8 mm diam., set of 10
1	ADAHCB602H	Precision Balance, 600 g /0,01g, with USB interface
1	665 025 4	Powder funnel, 100 mm diam., plastic
1	661 0771 8	Warning labels, GHS
1	667 050 4	Test tube rack, plastic, for 9 tubes, 18 mm Ø
1	664 043 4	Test tubes, Fiolax, 16 x 160 mm, set of 10
9	667 253 4	Rubber stopper solid, 14...18 mm Ø
1	666 966 4	Spoon-ended spatula polyamid, 180 mm
1	665 953 8	Dropping pipette 150 mm x 7 mm Ø, 10 pieces
1	666 584 4	Filtration stand for two funnels
1	665 954 4	Dropper bulb, 10 pieces
1	300 02 4	Stand base, V-shaped, small
1	300 42 4	Stand rod, 47 cm, 12 mm diam.
2	301 09 4	Bosshead S
2	666 555 4	Universal clamp 0...80 mm
1	670 8200 4	Petroleum ether, 90...110 °C, 250 ml Danger H225 H304 H315 H336 H411
1	672 1000 4	Glass wool, 10 g
1	674 8210 4	Sea sand, purified, 1 kg
1	670 0410 4	Acetone, 1 l Danger H225 H319 EUH066 H336
1	661 0581	Silica gel, 35-70 mesh, 1 kg
1	661 0821	Stopcock grease
1		additionally required: green leaves, dry or fresh

Experiment Instructions

PDF (Experiment description)

C2.4.4.1 Separation of a leaf extract with column chromatography

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Separation of a leaf extract with column chromatography

Description

Components of equipment sets

Experiment Instructions

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